Inhibition of Acrosin Activity with a Trypsin Inhibitor Blocks Human Sperm Penetration of the Zona Pellucida
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چکیده
منابع مشابه
Sperm Penetration of the Zona Pellucida of the Pig Egg.
In most mammals that have been studied, the first spermatozoon which penetrates an egg stimulates the egg to undergo a reaction—the 'zona reaction'—which bars further spermatozoa from penetrating through the zona pellucida (Braden, Austin & David, 1954). The sequel to the zona reaction may be classified into two categories, according to species: species in which spermatozoa are entirely prevent...
متن کاملHyperactivation of capacitated human sperm correlates with the zona pellucida-induced acrosome reaction of zona pellucida-bound sperm.
BACKGROUND The aim of this study was to determine the relationship between human sperm hyperactivation (HA), sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction (AR) of ZP-bound sperm in vitro. METHODS Sperm samples from 129 infertile men were studied. Motile sperm (2 x 10(6)) selected by Pure sperm were incubated with four oocytes in 1 ml human tubal fluid supplemented wit...
متن کاملSperm penetration through cumulus mass and zona pellucida.
Mammalian fertilization requires sperm to penetrate the cumulus mass and egg zona pellucida prior to fusion with the egg. Although sperm penetration through these physical barriers is essential, the molecular mechanism has not yet been completely elucidated. In addition to sperm motility, hyaluronan-hydrolyzing and proteolytic enzymes of sperm have been suggested to participate in the penetrati...
متن کاملHuman sperm degradation of zona pellucida proteins contributes to fertilization
BACKGROUND The mammalian oocyte extracellular matrix known as the zona pellucida (ZP) acts as a barrier to accomplish sperm fusion with the female gamete. Although penetration of the ZP is a limiting event to achieve fertilization, this is one of the least comprehended stages of gamete interaction. Even though previous studies suggest that proteases of sperm origin contribute to facilitate the ...
متن کاملProtease activity involvement in the passage of mammalian sperm through the zona pellucida.
The interaction between acrosome-reacted sperm and zona pellucida proteins is not yet fully understood. Serine protease acrosin and its zymogen proacrosin have been proposed to fulfill this function due to their capacity to bind zona pellucida glycoproteins. However, the molecular mechanism underlying this interaction has been merely speculative. Here we show that fucoidan (a sulfated polysacch...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1993
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod48.2.340